Abstract

PENA-LOPEZ, Brigitte O.; MARTINEZ-VEGA, Ruth A.  and  RINCON-OROZCO, Bladimiro. Real-time PCR standardization for detection and quantification of E7 oncogenes from six High-Risk Human Papillomaviruses. Rev. Univ. Ind. Santander. Salud [online]. 2023, vol.55, e66.  Epub Dec 04, 2023. ISSN 0121-0807.  https://doi.org/10.18273/saluduis.55.e:23047.

Cervical cancer is caused by persistent infection of the cervical epithelium with the high-risk genotypes of the Human Papilloma Virus (HR-HPV). For its detection, molecular tests are carried out that detect the L1 gene of HPV. This gene can be lost in up to 11 % of cases during the integration of viral DNA into the host genome, causing false negatives. On the other hand, the E7 oncogene is expressed during all stages of disease progression. The aim of this work was to standardize a real-time PCR of the E7 oncogene (E7-qPCR) for genotyping and quantification of 6 HR-HPV. The results show that the E7-qPCR amplified HPV-16, -18, -31, -33, -35 and -45 with high sensitivity with detection limits from 102 copies, efficiencies between 90 and 110 %, R2 values >0,97 and melting curve analysis revealing specific products.

Keywords : Papillomavirus infections; Cervical neoplasms; Genotyping; Real time polymerase chain reaction; E7 Oncogene.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )