Servicios Personalizados
Revista
Articulo
Indicadores
- Citado por SciELO
- Accesos
Links relacionados
- Citado por Google
- Similares en SciELO
- Similares en Google
Compartir
Revista de la Universidad Industrial de Santander. Salud
versión impresa ISSN 0121-0807versión On-line ISSN 2145-8464
Resumen
PENA-LOPEZ, Brigitte O.; MARTINEZ-VEGA, Ruth A. y RINCON-OROZCO, Bladimiro. Real-time PCR standardization for detection and quantification of E7 oncogenes from six High-Risk Human Papillomaviruses. Rev. Univ. Ind. Santander. Salud [online]. 2023, vol.55, e66. Epub 04-Dic-2023. ISSN 0121-0807. https://doi.org/10.18273/saluduis.55.e:23047.
Cervical cancer is caused by persistent infection of the cervical epithelium with the high-risk genotypes of the Human Papilloma Virus (HR-HPV). For its detection, molecular tests are carried out that detect the L1 gene of HPV. This gene can be lost in up to 11 % of cases during the integration of viral DNA into the host genome, causing false negatives. On the other hand, the E7 oncogene is expressed during all stages of disease progression. The aim of this work was to standardize a real-time PCR of the E7 oncogene (E7-qPCR) for genotyping and quantification of 6 HR-HPV. The results show that the E7-qPCR amplified HPV-16, -18, -31, -33, -35 and -45 with high sensitivity with detection limits from 102 copies, efficiencies between 90 and 110 %, R2 values >0,97 and melting curve analysis revealing specific products.
Palabras clave : Papillomavirus infections; Cervical neoplasms; Genotyping; Real time polymerase chain reaction; E7 Oncogene.