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Revista Facultad de Ingeniería

Print version ISSN 0121-1129

Abstract

ARBOLEDA, Alejandro; GONZALEZ, Julián; FRANCO, Manuel  and  VALLADARES, Liliana. In vitro comparison of cytocompotibility between Fibroin and polypropylene biomoteriols. Rev. Fac. ing. [online]. 2017, vol.26, n.45, pp.97-107. ISSN 0121-1129.  http://dx.doi.org/10.19053/01211129.v26.n45.2017.6056.

This study evaluates the growth of HeLa cells in vitro on fibroin and polypropylene. In order to determine cell proliferation in culture with these biomaterials, the number of cells/sample was obtained by metabolic reduction of 3-(4,5- dimetiltiazol-2-ilo)-2,5-difeniltetrazol Bromide, MTT assay, using direct and indirect evidence of cytotoxicity. For direct and indirect testing of cytotoxicity in fibroin and polypropylene materials, a statistical difference (p<0.005) was found in the average number of living cells in favor to the fibroin samples, regardless of the cytotoxicity test (i.e., direct or indirect), suggesting that the fibroin material allows for greater cell growth. The in vitro methods showed that fibroin material has better cell behavior in terms of viability, compared with polypropylene.

Keywords : Biomaterials; Cell Enlargement; Fibroin; Propylene Polymers; Silk.

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