Abstract

MENDOZA MEZA, Dary Luz  and  MALDONADO SANTANA, Humberto. Optimization a Methodology for the Isolation and the MolecularDetection of Toxocara Canis Eggs in Soil Samples. rev. cienc. [online]. 2015, vol.19, n.1, pp.41-51. ISSN 0121-1935.

Toxocariasis is a common zoonosis in developing countries, such as Colombia. Accidental ingestion of Toxocara sp. eggs represents the principal form of transmission in humans. Therefore, monitoring of environmental contamination is the better strategy for preventing the toxocariasis. The objective was to optimize a methodology for assessing soil contamination caused by Toxocara canis eggs. The soil samples were collected from a public park in the city of Barranquilla (Colombia). The Toxocara eggs were isolated by centrifugation/flocculation with MgSO4 (r = 1.20), ZnSO4(r= 1.18), NaCl (r= 1.18) and Sheather solution (r= 1.27). The parasite DNA was extracted and identified through amplification of ITS-2- DNAr (PCR-ITS-2) of T. canis and subsequent nucleotidesequencing. ANOVA analysis indicates a statistically significant difference (p <0.05) between the DNA concentrations obtained with the flocculation solutions used to isolate eggs. Tukey test results suggest that the modified Sheather´s solution is better for the flocculation. The PCR-ITS-2 analysis showed positive results for T. canis in 7 out of 13 samples examined (53.84 %). In conclusion, it is shown that the proposed method is efficient for the detection of T. canis in field samples

Keywords : Toxocara canis; toxocariasis; environmental contamination; ITS-2.

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