Abstract

VALVERDE MALAVER, Claudia Lorena; COLMENARES DULCEY, Ana Julia  and  ISAZA MARTINEZ, José Hipólito. Comparison of DPPH Free Radical Scavenging, Ferric Reducing Antioxidant Power (FRAP), and Total Phenolic Content of Two Meriania Species (Melastomataceae). rev. cienc. [online]. 2015, vol.19, n.2, pp.117-124. ISSN 0121-1935.

Searching for new antioxidants used in pharmaceuticals, cosmetics or agrochemicals have increased today. Many phenolic compounds have been reported as promising for this goal. Melastomataceae is rich in these compounds. Consequently, in this research the antioxidant power for Meriania nobilis and M. hernandoi ( Melastomataceae) was compared. Both species were extracted in acetone 70%. The latest was liquid-liquid partitioned in ethyl acetate (Mn or Mh 2.1), n-butanol (Mn or Mh 2.2) and aqueous fractions (Mn or Mh 2.3). Antioxidant activity was measured by DPPH free radical scavenging method (FRS50, mg.L-1), ferric reducing antioxidant power assay (FRAP, mg FeS04.7H20 (100 g)-1ES) and total phenolic content (TPC, mg AG g-1ES) by Folin-Ciocalteu. The present study revealed that Mh 2.1 and Mh 2.2 from M. hernandoi have a strong antioxidant power FRS50 = 5.71±0.085, FRAP = 53±1.5 and TPC = 224±7.1 and FRS50 = 4.22±0.001, FRAP = 65 mg±1.5, TPC = 240 ±8.6 respectively, as compared with positive control, quercetin DPPH FRS50 = 4.20±0.410, FRAP = 61±1.3. M. nobilis exhibited poor antioxidant power FRS50 = 286±1.5, FRAP = 2.01±0.079 and TPC = 26±1.5. This paper showed M. hernandoi as good source of antioxidant compounds with potential usage as cosmetic, pharmaceutical or agrochemical ingredients

Keywords : Antioxidant power; DPPH free radical scavenging method (FRS); ferric reducing antioxidant power assay (FRAP); Estimation of total phenolic content (TPC); Meriania nobilis; Meriania hernandoi.

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