Abstract

ORDONEZ BURBANO, Didier Erlinton et al. Biodegradation of Saturated Aliphatic Hydrocarbons by Microbes Isolated from Soils Polluted with Oil Derivatives. rev. cienc. [online]. 2018, vol.22, n.2, pp.33-44. ISSN 0121-1935.  https://doi.org/10.25100/rc.v22i2.7917.

The oil spills and their derivatives produce strong impacts on ecosystems balance, as they not only destroy their biodiversity, but also leave serious consequences that hinder their resilience. On the last decades, microbiology studies have shown evidences that microorganisms from polluted areas develop abilities to break pollutants. The purpose was to determine the ability of wild microorganisms from oil contaminated soil to biodegrade kerosene, two microorganisms strains was previously isolated from exposed to petroleum derivatives soil, which showed contaminant tolerance, was selected by means of minimum inhibitory concentration (MIC). The removal of saturated aliphatic hydrocarbons (n-alkanes of C-10 to C-16) were carried out with the strains selected and identified as Candida metapsilosis SH1 and Burkholderia cepacia SH3, which presented tolerance to concentrations greater than 10% of kerosene. The concentration of hydrocarbons was determined using a gas-mass chromatograph (GCMS) and the results were analyzed by comparing relative abundances. The microorganisms demonstrated the capacity for selective biodegradation of long chain hydrocarbons, causing fractionation correlated with the increase of short chain hydrocarbons (C10 to C12). These results show promising applications of the microorganisms tested to mitigate the contamination of sites affected by kerose and oil derivatives.

Keywords : alkane; bacterium; bioremediation; spill; yeast; kerosene..

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