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Revista Facultad de Odontología Universidad de Antioquia
versão impressa ISSN 0121-246X
Resumo
MONCADA, Gustavo et al. REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION (QPCR) FOR THE IDENTIFICATION AND QUANTIFICATION OF STREPTOCOCCUS MUTANS IN SALIVA AND DENTAL BIOFILM IN CHILDREN. Rev Fac Odontol Univ Antioq [online]. 2016, vol.28, n.1, pp.71-94. ISSN 0121-246X. https://doi.org/10.17533/udea.rfo.v28n1a4.
Introduction:
the objective of this study was to use real-time qPCR to identify and quantify the Streptococcus mutans species in samples of saliva and dental biofilm.
Methods:
27 children were randomly chosen with the following criteria: 8 years of age, low socio-economic levels, residing in the northern metropolitan area of Santiago de Chile; they were asked to attend an appointment while fasting with no teeth brushing for at least 12 hours, in order to collect non-stimulated saliva and a pool of supragingival dental biofilm of all the mesio-vestibular sides of anterior and posterior teeth. The amount of S. mutans in the samples was quantified by qPCR using primers that amplify a fragment of the gtfB gene of S. mutans.
Results:
the amplification showed 98% efficiency with a fluorescence of 3.36 cycles. The melting curve presented a single maximum at the same temperature for all samples.
Conclusion:
the methodology allows the specific identification and quantification of gene gtfB of S. mutans in saliva and dental biofilm in a quick and reliable manner, contributing to the identification of individual cariogenic risk.
Palavras-chave : S. mutans; qPCR; gtfB gen; dental biofilm; saliva.