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ORINOQUIA
versão On-line ISSN 0121-3709
Resumo
VALBUENA - VILLARREAL, Rubén D; ZAPATA-BERRUECOS, Beatriz E e CRUZ-CASALLAS, Pablo E. Induced breeding of Capaz (Pimelodus grosskopfii) with carp pituitary extract: preliminary report. Orinoquia [online]. 2010, vol.14, n.2, pp.133-139. ISSN 0121-3709.
In order to evaluate a protocol for induced breeding of Capaz Pimelodus grosskopfii, male and female in advanced stated of maturity were selected, it was evaluated in males by semen emission faced with a slight pressure in the coelomic cavity, and in females by the position of nucleus and oocyte diameter obtained through ovarian biopsy. As a final maturation inducer was used carp pituitary extract (CPE), with a single dose of 4 mg kg-1 for males and 5.75 mg.kg-1 (three doses) for females. Since the last dose of the female until ovulation, the water temperature was recorded to determine the latency time; the semen was taken 9 hours (h) after the last dose of the male. Females ovulated at 7 ± 0.2 hours on water temperature of 27.9 ± 0.2 °C. Reproductive fecundity was 14 800 ± 1000 ovocytes per female. Fertility was 90 ± 10 %, it was evaluated at 6 hours post fertilization (HPF) and the hatching rate of 80 ± 15 was observed at 12 HPF. These preliminary results permit to conclude that the CPE is effective in inducing final maturation of the species in captivity and therefore this becomes an alternative for diversification of pisciculture in Colombia.
Palavras-chave : Capaz; carp pituitary extract; induced breeding; Pimelodus grosskopfii.