Abstract

ORTIZ-MORENO, Martha L. et al. Evaluating microalgae Chlorella sorokiniana growth in different culture mediums in autotrophic and mixotrophic conditions. Orinoquia [online]. 2012, vol.16, n.1, pp.11-20. ISSN 0121-3709.

Chlorella sorokiniana is a microalgae having great biotechnological potential due to its ability to synthesise fatty acids having industrial interest, rapid growth and the ability to adapt to differing nutrient sources in both autotrophic and mixotrophic regimens, and even heterotrophic ones. This work was aimed at identifying the culture medium which would lead to maximum C. sorokiniana growth to enable its mass production. Sueoka, Guillard and Remital culture mediums (1, 2, 3, 4, 5g/L NPK fertiliser) were evaluated; they offer nutrients in autotrophic conditions and 30%, 50% and 80% concentration poultry manure leachate in a mixotrophic regimen. 86.5±0.75 x 10⁷ cells/ml maximum cell density and mean 0.3 generations/day specific growth rate were recorded in Remital medium. Tukey and Fisher statistical tests indicated that Remital was the best culture medium and that autotrophic conditions were ideal for C. sorokiniana growth. Using high concentrations of Remital did not significantly improve microalgae growth. Using low concentrations of Remital (1g/L) is thus recommended as the best option for mass production of C. sorokiniana biomass.

Keywords : Chlorella sorokiniana; cell density; poultry manure leachate; mixotrophy; autotrophy.

        · abstract in Spanish | Portuguese     · text in Spanish     · Spanish ( pdf )