Abstract

SARMIENTO-RUBIANO, Luz A  and  ESPINOSA-MEJIA, Meibys P. Developing and validating on HPLC-UV method for enlafaxine determination in serum. Orinoquia [online]. 2012, vol.16, n.2, pp.99-106. ISSN 0121-3709.

Venlafaxine is an antidepressant drug; it is asynthetic phenethylamine bicyclic derivative having antidepressant activity which consists of inhibiting serotonin, nor epinephrine and (to a lesser extent) dopamine reuptake in the presynaptic neuron, thereby promoting synaptic transmission in the central nervous system. Different methods have been reported for analysing venlafaxine in biological fluids, high-performance liquid chromatography (HPLC) being one of the most used to date. This study involved developing a rapid and simple method for determining venlafaxine in serum samples using liquid/liquid extraction, 229 nm UV detection, isocratic mobile phase and carbamazepine as internal standard. Linear (detection) range was 0.1 to 10 µg/ml (95% precision and accuracy) and 25 mintotal elution time per sample. This method was sufficiently sensitive, exact and reproducible for use in pharmacokinetic studies

Keywords : HPLC; venlafaxine; antidepressant; pharmacokinetic.

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