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ORINOQUIA

On-line version ISSN 0121-3709

Abstract

MENDEZ-LEAL, Diana C; GONGORA-ORJUELA, Agustín  and  GOMEZ-LEAL, Luz A. Isolating and identifying uterine bacterial flora in embryo-donor cows. Orinoquia [online]. 2014, vol.18, n.1, pp.86-91. ISSN 0121-3709.

Bacterial flora were isolated and identified in 21 embryo-donor cows from uterine washing when collecting embryos on day 7 post-insemination. Bacteria were identified by conventional methods based on phonotypical and morphological characteristics, development and biochemical and metabolic tests. The bacteria identified were Proteus mirabilis (4.8%), Archanobacterium pyogenes (4.8%), Klebsiella ozaenae (9.5%), E. coli (14.2%), Shiguella sonnei (23.8%), Bacilus spp. (33.3%), Pseudomona aeruginosa (52.3%) and Sthaphylococcus spp. (47.6%). A single bacterium was identified in 28.6% of the cows, two bacteria in 52.4% of the cows and three or more bacteria in 19% of them. The high bacterial diversity suggested various origins; new studies into their individual role and/or synergism regarding the development of uterine disease and possible effects on superovulatory response are needed.

Keywords : Uterine infection; bacteria; embryo transfer (DeCs).

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