Abstract

QUINTERO D., Juan C.; GUMERSINDO, FEIJOO C.  and  LEMA R., Juan M.. PRODUCTION OF LIGNINOLYTIC ENZYMES FROM BASIDIOMYCETE FUNGI ON LIGNOCELLULOSIC MATERIALS. Vitae [online]. 2006, vol.13, n.2, pp.61-67. ISSN 0121-4004.

White rot fungi produce a ligninolytic enzymatic complex with capacity to degrade a wide spectrum of toxic and recalcitrant substances as plaguicides, dyes, polyaromatic hydrocarbons, explosives, etc., which cause pollution in soils and water bodies. The grown-on soil fungi’s ligninolytic enzyme production and contaminant biodegradation are favorable when fungi are inoculated with a mixer of pieces of lignocellulosic material in soil. These materials provide carbon source to maintain growth and induce enzymatic complex. Fungi Bjerkandera adusta y Phanerochaete chrysosporium were employed to evaluated pieces of wood, corn cob and compost in order to produce ligninolytic enzymes manganese peroxidase (MnP) and lignine peroxidase (LiP). Pieces of wood provide an adequate support for production of MnP until 5.0 U/g dry material with Bj. adusta and 1.3 U/g dry material with P. chrysosporium. Corn cobs provide the best LiP activities. Ligninolytic enzymes only were detected in soil, when fungus was inoculated attached in these ligninolytic materials. These materials are appropriate for ligninolytic enzymes production and to inoculate fungus on contaminated soils.

Keywords : wood fungi; solid fermentation; ligninolytic enzymes; bioremediation; soil.

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