Abstract

GIL R, Juan F; MILTON, GÓMEZ B  and  TREJOS S, Juanita. Cytotoxicity and anticarcinogenic activity of two flavonoids isolated and purified from Brownea ariza Brenth. Vitae [online]. 2009, vol.16, n.1, pp.93-101. ISSN 0121-4004.

The growing resistance to anticancer agents has turned medical science and pharmacology towards finding new compounds with these characteristics; in this area the natural products have great relevance, since many of these have anticancer activity. Flavonoids as secondary metabolites have reported anti-tumor activity and can become important source for cancer treatment; why search isolate flavonoids Brownea ariza Brenth to evaluate their anticancer activity. The Phytochemical analysis of leaves of Brownea ariza Brenth. (Caesalpiniaceae) let isolate a flavonol identified as (1), and a glycoside flavonol described as Quercitrin (2). The structures are determined by spectroscopic techniques: UV-Vis with shift reagents; 1D and 2D NMR (¹H, ¹³C, COSY, DEPT, HMBC; HMQC), and mass spectrometry. It evaluates the cytotoxic activity in VERO cell (ATCC: CCL-81) and anticancer activity in Myeloma Murino SP2/0-Ag14 (ATCC: CRL-1581), doing count the number of viable cells by graduation with tripan blue, obtaining a concentration cytotoxic average (1) of 300 μg/mL and not determined to (2) in concentrations of work; anticancer activity for (1) has a median effective concentration of 150 μg /mL and not given to (2) in concentrations of work.

Keywords : Isolation; Purification; Flavonoids; Cytotoxicity; Anti-cancer agents; Brownea ariza.

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