versão impressa ISSN 0121-4004
PRIETO S, Gloria A; PEREA V, Janeth A e ORTIZ L, Claudia C. MICROBIAL BIOTRANSFORMATION OF (R)-(+)- LIMONENE BY Penicillium digitatum DSM 62840 FOR PRODUCING (R)-(+)-TERPINEOL. Vitae [online]. 2011, vol.18, n.2, pp. 136-172. ISSN 0121-4004.
Microbial biotransformation is a relevant strategy to obtain high added value natural compounds under controlled environmentally friendly conditions. In this research work, the biotransformation of (R)-(+)- limonene using Penicillium digitatum DSM 62840 was evaluated. The study variables were the following: culture medium, pH, microorganism growth phase, substrate concentration, and inducing effect of the substrate. The results showed that a concentration of 14.7 mM limonene in the medium named malt yeast broth at a pH of 3.5, inoculated with induced spores at the early stage of the exponential growth, produces a high specificity and the highest concentration (1864 mg/L) of (R)-(+)-α-terpineol. The product obtained has a considerable potential industrial application.
Palavras-chave : Bioconversion; biotransformation; monoterpenes; fungi; α-terpineol.