Abstract

BERNAL, CLAUDIA et al. PORCINE OSTEOCLAST CULTURE AND ITS BIOLOGICAL ACTION ON COMPOSITE SLIDES OF CARBONATED APATITE AND LOW DENSITY POLYETHYLENE (LDPE). rev.fac.med [online]. 2010, vol.18, n.1, pp.23-31. ISSN 0121-5256.

Bone tissue is in a continuous process of synthesis and resorption, functions that are performed by three main cell types: osteblasts, osteocytes and osteoclasts. Osteoclasts (OC) are big, multinucleate cells, producing carbonic anhydrase and tartrate-resistant acid phosphatase (TRAP). In continuation with previous works where CAp was synthesised by precipitation of aqueous solution and by solid state reaction, in this article the results of a culture of osteoclasts and its biological action over low-density polyethylene impregnated with carbonated apatite slides (LDPE-CAp) are shown. Osteoclast induction was achieved with the addition of specific inductors to cultures of porcine bone marrow progenitors, to which TRAP activity, detection of transcripts of rank, c-src and cat-K genes were determined. Cultures on LDPE-CAp slides analyzed with optical and electron microscopy demonstrated osteoclast activity and biodegradability of the ceramic, thus complementing previous studies on the osteoconductivity of this material in an in vivo porcine model.

Keywords : carbonated apatite; low-density polyethylene; osteoclasts; bone resorption.

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