Abstract

REY CUBILLOS, JORGE ARTURO et al. MECHANICAL STRESS AS OSTEOGENESIS REGULATOR IN HUMAN MESENCHYMAL STEM CELLS. rev.fac.med [online]. 2012, vol.20, n.1, pp.27-34. ISSN 0121-5256.

The role of mechanical stimulation for mesenchymal stem cells (MSCs) differentiation is a therapeutic alternative for applications in tissue engineering. The aim of this study was to evaluate the effect of mechanical strain on the differentiation and cellular mechanisms of mechanotransduction in MSCs. The cells were seeded in 75cm2 culture flasks and then exposed to uniaxial mechanical tensile strain of 500, 1000, 1500 and 2000 micro strains (με), 9 cycles / minute during 3 hours for 4 consecutive days. Runx2 and Sox9 transcription factors andOsteocalcin (OC), Collagen Type 1 (Col1) and Alkaline Phosphatase (ALP) gene expression was ascertained. After exposure to mechanical strain, osteogenic marker genes Col1, OC, and ALP were expressed temporally, while Runx2 and Sox9 transcription factors expression decreased, compared with control cells without stimulation, suggesting that mechanical stimulus induced differentiation of mesenchymal stem cells into osteoblast lineage. Identification of genes that translate mechanical stimuli in MSCs and modulate osteogenic differentiation hasimportant implications in regenerative medicine as an approach to functional tissue engineering.

Keywords : Mesenchymal stem cells; collagen type 1; osteocalcin; osteogenic differentiation; mechanotransduction.

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