Abstract

REY CUBILLOS, JORGE ARTURO; LAREO, LEONARDO; GUTIERREZ, SANDRA  and  GODOY CORREDOR, MARCELA. ALKALINE PHOSPHATASE (ALP) AND RUNX2 IN CELL CULTURES STIMULATED OSTEOBLASTS ELECTRIC FIELD. rev.fac.med [online]. 2012, vol.20, n.2, pp.14-20. ISSN 0121-5256.

The purpose of this study was to identify the electrical fields to be applied in osteoblast (Ob) cell cultures, in order to increase the expression of Alkaline Phosphatase (ALP) gene and the transcription factor Runx2. Ob cultured where from the American Type Culture Collection (ATCC) Ref CRL 11372. Cell Cultures received stimulation at day five, when they showed a confluent monolayer organization until day eight. The stimulus applied to each experimental group was 100mV, 200mV, 300mV, 400mV and 500mV respectively, a control group was cultured without stimulation. The electric field is generated with altern current (AC) and applied with two aluminum plates located parallel to 25cm2 culture flasks. The mRNA expression levels were measured by reverse transcription quantitative technique polymerase chain reaction (qRT-PCR). Aplication of AC increased expression of transcription factor RunX2 in direct proportion to the applied voltage. The ALP gene expression was inversely proportional to the stimulus. Electric field can increase or decrease the expression of genes that mediate the formation of bone tissue. Favoring differentiation of mesenchymal cells to Ob.

Keywords : osteoblasts; alkaline phosphatase; electrical stimulation; Runx2; gene expression.

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