Abstract

SARMIENTO, LUZ ADRIANA. LEVETIRACETAM DETERMINATION IN SERUM BY HPLC-UV. rev.fac.med [online]. 2014, vol.22, n.1, pp.12-19. ISSN 0121-5256.

Levetiracetam is an antiepileptic medicament derived from the pirrolidona, Its mechanism of action is different from other antiepileptic drugs, by binding specifically in the synaptic vesicle protein SV2A. Diversified methods have been brought to Levetiracetam analysis in varied biological fluids, being the High-Performance Liquid Chromatography (HPLC) one of the most used. In this study a method is described for rapid and simple Levetiracetam determination in serum samples with liquid - liquid extraction, ultraviolet detection to 205 nm, isocratic mobile phase and Captopril as internal standard. The method is linear over the studied range of 1 and 100 μg/ml, with 95% of precision and accuracy and total elution time to 18 min for a sample. This method is highly sensitive, exact and reproducible for be used in pharmacokinetic studies.

Keywords : HPLC; Levetiracetam; antiepileptic; pharmacokinetic.

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