Resumo

DAZA, CARLOS; GUILLEN, JAVIER; REY, JORGE  e  RUIZ, VALENTINA. EVALUATION OF A METHOD OF EXTRACTING AND PURIFYING DNA FROM MUSCLE TISSUE FIXED IN FORMALDEHYDE UNIDENTIFIED BODIES. rev.fac.med [online]. 2014, vol.22, n.1, pp.42-49. ISSN 0121-5256.

Obtaining human DNA in large quantity and high purity from muscle tissue fixed in formaldehyde unbuffered is of utmost importance in amplification by Chain Reaction (PCR) for application in identification studies and genetic affiliation. In this study, the efficiency of DNA FFPE TISSUE QIAampR Kit and a modification thereof based washes with PBS and the time of digestion with proteinase K, compared to the amount and quality of the nucleic acid were evaluated. Differences were significant between the exposure times with proteinase K (PK) in relation to the quantity and purity of the product prior washings muscle tissue extraction. These results provide a guideline for the design of experiments according to the effect of fixing, optimizing human resources and inputs.

Palavras-chave : Formaldehyde muscle tissue; proteinase K; cell washing.

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