Abstract

ARANGO ROMERO, Paola Andrea et al. Antioxidant activity of non-psychoactive cannabis varieties from North Cauca, Colombia. Ciencia en Desarrollo [online]. 2024, vol.15, n.1, pp.178-193.  Epub Oct 15, 2024. ISSN 0121-7488.  https://doi.org/10.19053/01217488.v15.n1.2024.15214.

The present study aims to publicize the chemical composition and possible medicinal potential of varieties of non-psychoactive cannabis grown in the department of Cauca. Cannabinoids were identified and quantified by high-performance liquid chromatography coupled to an ultraviolet detector (HPLC/UV) for the analysis of the flower, and gas chromatography coupled to a mass spectrometer (GC-MS) for the analysis of the ethanolic extracts and content terpenes. Phenols were quantified by reaction with the Folin & Ciocalteau reagent; for the determination of flavonoids and anthraquinones, the extracts were treated with AlCl3. Finally, to determine the antioxidant activity, three methods were used: DPPH, ABTS and FRAP. It was possible to determine that varieties A and B contained percentages of total tetrahydrocannabinol (THC) less than 1 % and percentages of total cannabidiol (CBD) between 9-15 %. In the ethanolic extracts concentrations (w/w) of CBD were reached in varieties A and B, of 10% and 13.7 %, respectively. Nine terpenes from sample A and seven from sample B were identified and quantified, with β - caryophyllene being the most abundant in both. Considering that there is evidence in the literature that the CBD/THC ratio influences biological activity, ethanolic extracts of varieties A and B are expected to have moderate to low antioxidant activity, which, according to some researchers, it may be associated with the neuroprotective effect, which may be favored by the presence of β - caryophyllene.

Keywords : Antioxidant activity; Cannabinoid; Cannabis; Terpenoid.

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