Abstract

JURADO-GAMEZ, Henry  and  CERON-CORDOBA, Jhon Fredy. Effect of microencapsulated Lactobacillus lactis on Klebsiella pneumoniae under in-vitro gastrointestinal conditions. Ciencia en Desarrollo [online]. 2024, vol.15, n.2, pp.1-7.  Epub Dec 11, 2024. ISSN 0121-7488.  https://doi.org/10.19053/uptc.01217488.v15.n2.2024.17201.

K. pneumoniae is found natively in the respiratory and gastrointestinal tracts of humans, domestic and wild animals with possible zoonotic links. It is commonly associated with nosocomial infections and has been reported in some foods as a vector of transmission. Lactobacillus are considered a preventive alternative to the use of antibiotics in animal and human health, thanks to the production of lactic acid, organic acids, exopolysaccharides (EPS) and secondary metabolites with antibacterial, antioxidant, rheological and food preservation properties. The objective of the research is to evaluate the inhibitory potential of Lactobacillus lactis microencapsulated by spray drying on Klebsiella pneumoniae under gastrointestinal conditions in-vitro. Preliminary tests were conducted, including growth at different temperatures (37°C and 42°C), gas production, and catalase activity. Subsequently, fermentation kinetics were performed, evaluating CFU/mL, sugar consumption (mg/L), protein production, pH, and lactic acid production. Microencapsulation was carried out through spray drying with inulin (5%) and maltodextrin (5%), and the resulting product was stored for 50 days. Structural, stability, and survival studies under in-vitro gastrointestinal conditions were conducted after the storage period. Finally, inhibition tests of L. lactis on K. pneumoniae were performed. The results allow us to conclude that there is a protective effect on microencapsulated L. lactis and an inhibitory response of the lactic strain on K. pneumoniae.

Keywords : Klebsiella pneumoniae; Lactobacillus; microencapsulation; human health; animal health; zoonosis.

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