Abstract

PEDROZA DIAZ, Johanna et al. Proteomic analysis of cerebrospinal fluid of neuropsychiatric patients: An initial approach to the search for biomarkers. Rev.Colomb.Reumatol. [online]. 2014, vol.21, n.3, pp.115-124. ISSN 0121-8123.

Introduction: The diagnosis of neuropsychiatric lupus (NP-SLE ) is based on clinical features using the nomenclature and description of case of the American College of Rheumatology, 1999. However, the lack of specificity of these syndromes makes the diagnosis difficult. Objective: To identify molecules in CSF protein profiles of NP-SLE patients with differential expression, which could be used as biomarkers for diagnosis. Methodology: A total of 26 CSF samples from five groups, one NP-SLE and four control groups were used. The tools used for the execution of this work included two-dimensional electrophoresis to obtain protein profiles, ImageMaster 2D Platinum software for comparative analysis, and statistical analysis using Graphpad Prism Version 6.0. Results: Statistical analysis and comparison revealed three spots with differential expression when comparing SLE patients with NP-other study groups. Discussion: The techniques used for the implementation of this proposed research allowed the identification of a line of study for finding biomarkers in neuropsychiatric lupus, which could be used as a diagnostic / prognostic disease support.

Keywords : Biomarkers; Proteomics; Cerebrospinal fluid; Neuropsychiatric lupus.

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