SciELO - Scientific Electronic Library Online

 
vol.12 número2BATS DIVERSITY IN FOUR TOWNS OF COASTAL AREA IN CÓRDOBA DEPARTMENT-COLOMBIAPREVALENCE OF BACTERIA ASSOCIATED WITH INFECTIOUS INFERTILITY IN BOVINES OF MONTERIA, COLOMBIA índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Em processo de indexaçãoCitado por Google
  • Não possue artigos similaresSimilares em SciELO
  • Em processo de indexaçãoSimilares em Google

Compartilhar


Revista MVZ Córdoba

versão impressa ISSN 0122-0268versão On-line ISSN 1909-0544

Resumo

ARIAS L, Catalina; RUIZ, Tatiana; OLIVERA A, Martha  e  TARAZONA, Ariel. EFFECT OF SUPPLEMENTATION WITH ALANINE AND GLICYNE ON THE INITIAL CLEAVAGES OF BOVINE EMBRYOS PRODUCED IN VITRO. Rev.MVZ Cordoba [online]. 2007, vol.12, n.2, pp.1020-1027. ISSN 0122-0268.

Objective. To determine the effect of supplementation with alanine and glicyne in vitro culture systems on the cleavage rate of bovine embryos under conditions of high (20%) and lower (7%) oxygen tension. Materials and methods. The embryos were produced from oocytes matured in vitro in M-199 supplemented with hormones and inseminated with cryopreservated semen; the zygotes were cultivated in CR1-AA medium. At culture moment alanine and glicyne were added (5 mm and 10mM final respectively). Four (4) treatments were used (T1: amino acids and low oxygen tension; T2: amino acids and high oxygen tension; T3: non amino acids and low oxygen tension; T4: non amino acids and high oxygen tension). The cleavage was evaluated to 48 hpi (hours post insemination) being evaluated the number of embryos cleavaged on the total of culture embryos and the development stage (non cleavaged, 2, 4, 5-8 cells). The statistical program STATISTICA version 5.0 was used. Results. Embryos rate of 5-8 cells in the treatment one regarding the other 3 ones were higher (p = 0.007). Conclusions. The amino acids alanine, glicyne and in low oxygen tensions are fundamental for the initial cleavages because the proportion of competent embryos increases up to 48 hpi.

Palavras-chave : Cleavage; bovines; embryos; amino acids.

        · resumo em Espanhol     · texto em Espanhol     · Espanhol ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons