SciELO - Scientific Electronic Library Online

 
vol.12 issue2AGRONOMIC EVALUATION OF 25 GENOTYPES OF MAIZE (Zea mays) for FORAGE PURPOSES IN THE HALF SINU VALLEYPANCYTOPENIA IN A CANINE CAUSED FOR ADMINISTRATION OF ESTROGENS TO AVOID A NOT WANTED PREGNANCY author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • On index processCited by Google
  • Have no similar articlesSimilars in SciELO
  • On index processSimilars in Google

Share


Revista MVZ Córdoba

Print version ISSN 0122-0268On-line version ISSN 1909-0544

Abstract

LA³PEZ, Angela; OLIVERA, Martha; RUIZ, Tatiana  and  TARAZONA, Ariel. CO-CULTURE EFFECT ON EARLY DEVELOPMENT OF IN VITRO BOVINE EMBRYOS. Rev.MVZ Cordoba [online]. 2007, vol.12, n.2, pp.1061-1067. ISSN 0122-0268.

Objective. To study the effect of co-culture with oviductal cells on the cleavage rate 48 hours post insemination (hpi) of bovine embryos in low oxygen tensions. Materials and methods. Slaughterhouse ovaries were collected for the extraction of oocytes put on TCM 199 medium supplemented with hormones, fertilized with semen cryo-preserved and co-cultured in CR1aa medium with oviductal cells during 48 hours. Total cleavage rate and cleavage per stages of 2-4 cells and 5-8 cells were evaluated. The viability of cells for the co-culture was determined by observation of the ciliary movement and monolayer observation. Treatments were T1: oviduct cells + oxygen 20%; T2: oviduct cells + oxygen 7%; T3 and T4 were controls without cells for both oxygen concentrations. Results. For cleavage rate there was not significant difference among the four treatments, but there was a tendency to more cleavage for co-cultured embryos with cells and 20% oxygen. Conclusions. The use of high oxygen rates (20%) in co-culture systems with oviductal cells intend to improve the cleavage percentages to 48 hpi.

Keywords : Co-culture; bovines; embryos; oviductal cells.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License