Abstract

RINCON F, Juan; LOPEZ H, Albeiro  and  ECHEVERRI Z, Julian. Structure and genetic diversity of a population Holstein cows of Antioquia department, using a polymorphism of bGH gene. Rev.MVZ Cordoba [online]. 2013, vol.18, n.1, pp.3346-3354. ISSN 0122-0268.

Objective. To determine the allele frequencies and genotypic polymorphism of the intron 3 of gene bGH and estimate structural parameters in Holstein cattle populations. Materials and methods. The study was conducted with 1366 Holstein cows belonging to 120 herds in 11 municipalities of the department of Antioquia. DNA was extracted by the Salting out method and genotyping was carried out using PCR-RFLP. Genetic diversity was determined by comparing the heterozygosities, Hardy-Weinberg (HW) and genetic differentiation between populations was performed using the Arlequin software 3.0. The allelic and genotyping frequencies were assessed using the SAS statistical software. Results. The genotype frequencies found were 0.764 and 0.013 (+/-) 0.223 (+/+), (-/-) and allele frequencies were 0.876 (+) and 0124 (-). There was no unbalance for Hardy Weinberg in the subpopulations. The genetic diversity determined by comparison of the heterozygosity was low among populations but within them it was not. The FST value of the entire population was 0.0068 and significant (p<0.05), FST also matched some were values ranging from 0.0 to 0.13. The statistical FIT and FIS were not significant. Conclusions. The gene bGH is an interesting candidate to economically evaluate important traits because it does not seem to have been subject to direct selection, has a mean variability in populations, showing significant genetic differentiation between several municipalities, resulting from the different production systems and access to biotechnologies.

Keywords : Dairy cattle; molecular markers; polymorphism; population genetics.

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