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Revista MVZ Córdoba
Print version ISSN 0122-0268
Abstract
RAMIREZ M, Juan; CIFUENTES C, Cristian; PARRADO, Yinet and AVILES B, Mónica. Evaluation of induced breeding of Pimelodus blochii nicuro (telostei, pimelodidae) using different inducing hormone. Rev.MVZ Cordoba [online]. 2013, vol.18, n.2, pp.3512-3517. ISSN 0122-0268.
Objective. Evaluate the effect of two hormonal inducers in induced breeding of nicuro Pimelodus blochii. Materials and methods. The experimental process used sexually mature adults exposed to three intramuscular treatments, a single dose of 0.25 mL/g Ovaprim® (OVAP) (T1), 0.5 mL/kg of OVAP (T2) and 6.25 mg/kg of pituitary carp extract (EPC) (T3), for the latter treatment the injection was divided into 20 and 80%, with an interval of 12 hours between applications. Prior to the removal of gametes, the animals were tranquilized by immersion in a Tricaine Methanesulfonate (90 mg/L) solution. Reproductive performance was evaluated using ovulation rates (females ovulated/treated females), fertility (Fa) (eggs/female), relative fertility (Fr) depending on the number of eggs spawned per gram of weight. The dry method of fertilization was used and the fertility rate was determined six hours post-fertilization (HPF). Results. The Ovulation (°h) was: for T1 297.1±30.0, T2 294.6±32.9 and T3 247.3±13.1°h. Ovulated females were obtained in all treatments, where the highest rates of ovulation were obtained with Ovaprim ® (T1 and T2) with 36.4 and 50% respectively. Fertilization rates were higher than 50% for treatments 1 and 2, with values of 74.5 and 32.7% respectively. Conclusions. The use of hormonal inducers may be effective to ensure nicuro induced reproduction with a single dose of 0.25 and 0.5 mL/kg Ovaprim®.
Keywords : Gametes; Pimelodus blochii; sexual reproduction.