Abstract

LENIS S, Yasser; OLIVERA A, Martha  and  TARAZONA M, Ariel. Linoleic acid effect on PGF₂a and PGE₂ prostaglandin production in endometrial cells. Rev.MVZ Cordoba [online]. 2013, vol.18, n.2, pp.3559-3568. ISSN 0122-0268.

Objectives. Evaluate the in vitro effect of AL on the production of PGF2a and PGE₂ in bovine epithelial endometrial cells (CEEP). Materials and methods. Isolated CEEP were cultured and supplemented with AL at different concentrations (1µM, 10µM, 100µM), oxytocin (OT) (0.1 µM) and bovine interferon (BINT-τ) (50 ng / ml). PGF2a and PGE₂ were quantified as response variables at different times. In the control, the concentration of PGF2a in the supernatant increased over time and was 1.2 times greater than the production of PGE₂. Results. AL reduced the concentration of PGF2a (p <0.05) in the supernatant, and did not affect (p> 0.05) the production of PGE₂. The combined effect of LA and OT in the production of PGF2a is different in time, LA partially inhibited the stimulatory effect of OT in the production of PGE₂, the joint effect of LA and BINT-τ increased (p<0.05) when this inhibition at 24h. Conclusions. LA alone negatively affects the concentration in the supernatant of PGE₂ in time. Regarding PGE₂ , LA alone does not affect the concentration in the supernatant.

Keywords : Endometrium; interferons; luteolysis; maternal; oxytocin.

        · abstract in Spanish     · text in Spanish     · Spanish ( pdf )