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Revista MVZ Córdoba
versão impressa ISSN 0122-0268
Resumo
ARJONA J, Guadalupe et al. Antibodies of Trypanosoma cruzi, Leishmania mexicana and Leishmania braziliensis in domiciled dogs in Tabasco, Mexico. Rev.MVZ Cordoba [online]. 2017, vol.22, n.2, pp.5829-5836. ISSN 0122-0268. https://doi.org/10.21897/rmvz.1011.
Objective.
To determine Trypanosoma cruzi (T. cruzi), Leishmania mexicana (L.mexicana) and Leishmania braziliensis (L.braziliensis) circulating antibodies in dogs from Chontalpa region in Tabasco, Mexico using ELISA diagnostic techniques Fe-SOD and Western blot.
Materials and methods.
For this study, 119 serums were obtained from domiciled dogs. Serums were tested for antibodies against T. cruzi, L. mexicana and L. braziliensis, using ELISA and Western Blot sod as diagnostic test. The antigenic fraction used in both tests was the Fe-SOD excreted by the species of Leishmania and Trypanosoma.
Results.
The obtained frequency in this study was 3.36% for T. cruzi, 9.24% for L. mexicana and 10.08% for L. braziliensis.
Conclusions.
The present study has demonstrated the presence of antibodies to these parasites in Chontalpa region from Tabasco, Mexico.
Palavras-chave : Antibodies; blood serum; enzyme - linked inmunospot assay; Western blotting.
