Abstract

NEIRA F, Laura Fernanda; VERA A, Angélica María  and  ESCOBAR, Patricia. Genotoxiciyt from Nifurtimox in comet assay. Revista médica Risaralda [online]. 2016, vol.22, n.1, pp.3-8. ISSN 0122-0667.

Nifurtimox is a synthetic 5-nitrofuran used in treating Chagas disease and potentially genotoxic in experimental models. The aim of this study was to determine the cell toxicities and DNA damage caused by nifurtimox on Vero, J774, NIH/3T3 and THP-1 cells. Vital trypan blue staining and MTT colorimetric test for cell toxicities and alkaline comet assay for DNA damage were performed. Comets were counted under fluorescent microscope and the % of DNA damage was calculated and scored from 0 (no damage) to 4 (high damage). J774 cells was the more sensitive and THP-1 cells the less one with values of CC50 34.04-138.58 µg/ml and CC9o 130.58->300 µg/ml. At a dose of 100 µg/ml of nifurtimox, DNA damage was 79.75, 85.30 % and 10.25% on NIH/3T3, J774 and THP-1 cells respectively. Vero cell showed 80% DNA damage in both treated and untreated cells. Both, cell toxicity and DNA damage were dependent on cell type and drug concentration. It is important to take these variables into account for final conclusions when these types of assays, especially comet assay are performed.

Keywords : nifurtimox; cytotoxicity; genotoxicity; comet assay; cell lines.

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