Resumo

BEDOYA, Ingrid Johanna et al. Qualitative comparison between different cultures media and conditions for primary isolation of Helicobacter pylori obtain of gastric biopsies of dyspeptic patients. Revista médica Risaralda [online]. 2017, vol.23, n.2, pp.29-33. ISSN 0122-0667.

Helicobacter pylori cultivation is essential to study the sensitivity of isolates to various antimicrobial agents, diagnostic testing and evaluating toxicity and virulence also preserve isolates with future research purposes. Under optimum conditions, cultivation has a sensitivity approaching 90% and a specificity of 100%, but the rates of isolation of infected individuals may vary between 23.5% to 97%, depending on a number of factors such as components culture medium, transporting biopsies, self-medication with proton-pump inhibitor / antibiotics and methods of making the biopsy. For this reason, the aim of the research study was to compare different culture media and conditions of transport, handling and processing of biopsies for the isolation of Helicobacter pylori. 27 antrum's gastric biopsies and body 's gastric biopsies obtained from dyspeptic patients were analyzed; were seeded for duplicate on three different culture's mediums designated as A, B and C and under two culture's conditions. The biosies were seeded for Printing (Touch) in the endoscopy unit and maceration in the microbiology's laboratory. The biopsies were transported in transport medium with and without supplements and antibiotics. The results of the recovery rate in the culture medium A was 59.2%, in the B was 37% and in the C was 18.5%. In the culture médium A and B were observed a vigorous growth of H. pylori, otherwise in the culture medium C, the colonies were not observed as bright. Seeding by maceration in laboratory culture medium and A provided the best conditions for recovery of H. pylori.

Palavras-chave : Helicobacter pylori; culture medium; biopsy; gastritis; stomach; dyspepsia.

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