Abstract

ORTIZ, Ángela et al. "in vitro" evaluation of the probiotic capacity of a native strain of Saccharomyces cerevisiae. Univ. Sci. [online]. 2008, vol.13, n.2, pp.138-148. ISSN 0122-7483.

The in vitro probiotic capacity of a native strain of Saccharomyces cerevisiae (A) was evaluated and compared with a commercial strain (B) used as a probiotic. The effect of the concentration of sugarcane molasses (10, 20 and 30% (w/v)) on the biomass production was investigated and kinetic parameters were determined. The best molasses concentration was 20% (w/v) and differences in biomass production on molasses medium between strain A (28 g/L) and control strain B (3 g/L) were observed. In vitro tests such as tolerance to bile salts, pH and gastric juices were carried out, and no differences in growth between strain A and B were found. Cholesterol reduction on presence of bile salts after 12 hours of incubation was of 54% for strain A and 58% for strain B. Both strains showed adherence to Caco-2 cells. Results reveal that strain A possesses in vitro probiotic properties that can be verified with further in vivo studies to confirm its suitability as probiotic in animal nutrition.

Keywords : Adherence; Biomass; Sugarcane molasses; Probiotic; Saccharomyces; Tolerance.

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