Print version ISSN 0122-7483
CHAVEZ-GARCIA, Mónica et al. Effects of substrate and light exposition in the production of Trichoderma sp.. Univ. Sci. [online]. 2008, vol.13, n.3, pp. 245-251. ISSN 0122-7483.
In order to evaluate the effect of temperature and light conditions on biomass production of a Trichoderma sp. strain, three culture media were tested: rice 53% (w/w), rice 53% (w/w) -molasses 3% (w/w) and rice 53% (w/w)-molasses 10% (w/w) in distilled water. Incubation conditions were: 25°C, constant light and a photoperiod of 24 h light/24 h darkness during 8 days. The evaluated parameters were population density (conidia/mL), spore germination after 24 hours and purity percentage. The results showed that solid fermentation using rice - distilled water as substrate at 25°C and constant light, allowed the highest conidia yield (45x1018 conidia/mL), 96% germination after 24 hours, and 92.1% purity. The liquid fermentation rendered a purity of 76.8% and conidia germination of 91.2% after 24 hours, showing a disadvantageous lower purity percentage compared to solid fermentation.
Keywords : rice; biocontroller; sporulation; germination; solid fermentation.