Print version ISSN 0122-7483
ARENAS-CARVAJAL, Raúl; PACHON-GOMEZ, Erica; MENDEZ-CALLEJAS, Gina and GUZMAN-AVENDANO, Antonio. Study of the inhibitory effect of Salvia scutellarioides extracts on the activity of the angiotensin-converting enzyme. Univ. Sci. [online]. 2009, vol.14, n.2, pp. 141-150. ISSN 0122-7483.
Objective. To identify groups of secondary metabolites of Salvia scutellarioides present in the fraction with the greatest inhibitory effect on the activity of the angiotensin-converting enzyme (ACE). Materials and methods. Dry plant material was used to prepare ethanolic extracts that were then concentrated and separated by column chromatography using solvents of different polarity (petroleum ether, dichloromethane, diethyl ether and ethanol). Subsequently, lung tissue isolated from Wistar rats was broken and centrifuged in order to separate the soluble material. Proteins found in the supernatant were separated using a Sephacryl column; a pool was made with the fractions that showed activity with hippuryl-L-histidyl-L-leucine (HHL), the substrate of the ACE. This enzyme extract was used to measure the effect of plant extracts obtained from S. scutellarioides on the ACE activity. Results: The fraction of ethyl acetate (T2) showed a greater inhibitory effect on the ACE activity. Metabolites found in T2 fraction were: tannins, cardiac glycosides, coumarins, and quinones. Conclusion. An antihypertensive effect was found for the plant species Salvia scutellarioides by studying the inhibitory effect on the ACE activity. Several groups of secondary metabolites present in the T2 fraction were identified, which could be responsible for this effect.
Keywords : angiotensin II; angiotensin-converting enzyme; arterial hypertension; enzymatic activity; Salvia scutellarioides.