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Ciencia y Tecnología Agropecuaria
Print version ISSN 0122-8706
Abstract
MORENO-GALVAN, Andrés; ROJAS-TAPIAS, Daniel F. and BONILLA, Ruth. Development and evaluation of an alternative culture medium for mass cultivation of Azospirillum brasilense C16 using sequential statistical designs. Corpoica cienc. tecnol. agropecu. [online]. 2012, vol.13, n.2, pp.201-206. ISSN 0122-8706.
For mass plant growth-promoting inoculant production, a high-yield culture medium is fundamental. Sequential application of statistical designs was used to optimize Azospirillum brasilense C16 biomass production. Six nutritional (glycerol, glutamate, mannitol, citric acid, yeast extract and K2HPO4 3H2O) and three mineral sources (MgSO4 7H2O, FeCl3 and NaCl) were evaluated using five statistical experiments Placket-Burman, factorial design, steepest ascent, response surface analysis, and mineral screening. The optimum medium composition (g L-1) was as follows: 28.33 glutamate, 2.92 yeast extract, 1.34 K2HPO4 3H2O, 0.5 MgSO4 7H2O and 0.02 FeCl3. After 24 hours of incubation, protein (32.04 mg) and dry biomass (1.51 g L-1) were 1.72 and 1.68 times higher than in conventional growth medium.
Keywords : culture medium optimization; factorial designs; culture medium design; bioinoculant production.