Abstract

PEREZ MONCADA, Urley Adrian; RAMIREZ GOMEZ, María Margarita; ZAPATA NARVAEZ, Yimmy Alexander  and  CORDOBA SANCHEZ, Juana Marcela. Effect of the single and combined inoculation with Arbuscular Mycorrhizal Fungi (AMF) and Plant Growth Promoting Rhizobacteria (PGPR) in micropropagated blackberry plants (Rubus glaucus L.). Corpoica cienc. tecnol. agropecu. [online]. 2015, vol.16, n.1, pp.95-103. ISSN 0122-8706.

The aim of this study was to obtain blackberry seedlings of three ecotypes of blackberry (monterrico, sin espinas and castilla), from in vitro cultures inoculated individually and combined with Arbuscular Mycorrhiza Fungi (AMF) Glomus sp. (GEV02) and plant growth promoting rhizobacteria strains of Pseudomonas migulae (Pf014) and Bacillus amyloliquefaciens (Bs006). The growth variables were aerial and root length (cm), leaf and root fresh and dry weight (g), root volume (cm³) and leaf area (cm²). The symbiotic variables were root colonization (%) by the AMF. The results show a possible synergism between Glomus sp. (GEV02) and rhizobacteria evaluated in combination as these showed the highest values in all variables analyzed. Using the mixture of these organisms a better establishment, development and seedling vigor of default in the three ecotypes was observed, improving survival (≥80 %) in the stages of hardening and acclimatization.

Keywords : Arbuscular Mycorrhizal Fungi (AMF); Plant Growth Promoting Rhizobacteria (PGPR); Rubus glaucus; in vitro culture.

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