Abstract

SALAZAR MERCADO, Seir Antonio; BOTELLO DELGADO, Edinson Alexander  and  QUINTERO CALENO, Jesús David. Tetrazolium test optimization to evaluate the viability in Solanum lycopersicum L. seeds. Cienc. Tecnol. Agropecuaria [online]. 2020, vol.21, n.3, e1344.  Epub Aug 31, 2020. ISSN 0122-8706.  https://doi.org/10.21930/rcta.vol21_num3_art:1344.

Tomato (Solanum lycopersicum) is one of the most consumed vegetables worldwide with increasing demand. Therefore, knowing its seeds viability is of utmost importance since these are the basis of their production success. Accordingly, the study aimed at optimizing the tetrazolium test by determining the appropriate pretreatment to enhance it. Plant material was collected from crops established in the municipality of Cáchira, department of Norte de Santander, Colombia. The seeds were extracted from ripe fruits and subsequently exposed to preconditioning with sodium hypochlorite and distilled water for 10 minutes, with three concentrations of 2,3,5-triphenyl tetrazolium chloride (0.25%, 0.15%, and 0.10%) and different exposure times (6 h, 12 h, and 24 h). The viability data obtained were corroborated by the germination test on wet paper towels. The viability results with the highest correlation with the germination test were obtained using the 0.25% and 0.15% concentrations, and utilizing the pre-conditioning treatment with sodium hypochlorite as well as immersion in distilled water.

Keywords : pretreatment; seed germination; seed quality; tomato; triphenyl tetrazolium chloride.

        · abstract in Spanish     · text in English | Spanish     · English ( pdf ) | Spanish ( pdf )