Resumo

CAETANO NUNES PAZDIORA, Bruna Rafaela et al. A NON-INVASIVE METHOD FOR EXTRACTING DNA FROM CORYPHAENA HIPPURUS. Bol. Invest. Mar. Cost. [online]. 2015, vol.44, n.2, pp.395-399. ISSN 0122-9761.

A protocol for DNA extraction and effect of the type of tissue material for obtaining DNA from dolphinfish in high quantity and quality for molecular studies was evaluated. The DNA of 25 individuals was extracted from the digestion of the muscle and the dorsal fin, with proteinase K, and the extraction kit for blood and animal tissue. DNA were obtained in all the samples; however, dorsal fins samples showed significantly higher amount of DNA than muscle samples. The definition of an appropriate methodology of DNA extraction can contribute to studies of genetic structure of the species. DNA extraction from dolphinfish dorsal fins, non-invasive method, is an alternative that is environmentally, socially, and commercially viable, where sample collection does not require the sacrifice of the animal and do not alter its economic value.

Palavras-chave : Dorsal fin; DNA; dolphinfish.

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