Resumen

GALVIS GARCIA, Jhon Henry; OCAMPO, Diana Marcela; OCAMPO, Rogelio  y  GUTIERREZ-CARDENAS, Paul David A.. TOXIC ACTIVITY OF ANNONA CHERIMOLIOIDES (ANNONACEAE) STEAM BARK EXTRACTS ON ARTEMIA SALINA. Bol. Cient. Mus. Hist. Nat. Univ. Caldas [online]. 2012, vol.16, n.2, pp.17-22. ISSN 0123-3068.

The Annonaceae family is characterized by the presence of many bioactive substances from different chemical nature. Alkaloids, flavonoids and acetogenins have been characterized and reported in this family. The bioactivity of this type of Annonaceae plant metabolites is associated with its effect as insecticidal, antitumoral, antibacterial, antimalarial, leishmanicidal, anthelmintical and cytotoxical properties. Since 1982, bioassays have been developed for the determination of cytotoxicity with the use of "Brine Shrimp" (Artemia salina), which is used for toxical screening of high toxicity extracts. This research aims to evaluate the toxic activity of different polarity extracts obtained from Annona cherimolioides stem bark. Column chromatography and preparative layer chromatography were applied for extraction and isolation of the alkaloids present in the Annona cherimolioides stem bark. Extracts, fractions and alkaloidal type purified compounds were tested in vivo on Artemia salina to determine cytotoxic activity. A purified compound was obtained, in which the presence of an aporphine nucleus was obtained according to nuclear magnetic resonance 1-H (NMR 1-H) and spectrophotometry. The crude extract showed higher toxicity on Artemia salina (< 250 ppm), due to the synergism of the alkaloids found in this extract and the pharmacological properties attributed to the aporphine nucleus present in extracts, fractions and purified compounds.

Palabras clave : Alkaloids; Annona cherimolioides; Artemia salina; toxicity.

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