Abstract

ORTIZ PAZ, Rocío Alexandra; GUZMAN PIEDRAHITA, Óscar Adrián  and  LEGUIZAMON CAYCEDO, Jairo. IN VITRO EFFECT OF Purpureocillium lilacinum (Thom) Luangsa-Ard et al. AND Pochonia chlamydosporia var. catenulata (Kamyschko ex Barron & Onions) Zare & Gams ON THE ROOT-KNOT NEMATODES [P. chlamydosporia (Kofoid & White) Chitwood AND Meloidogy nemayaguensis Rammh & Hirschmann]. Bol. Cient. Mus. Hist. Nat. Univ. Caldas [online]. 2015, vol.19, n.2, pp.154-172. ISSN 0123-3068.  https://doi.org/10.17151/bccm.2015.19.2.9.

Purpureocillium lilacinum strain PL-11 and Pochonia chlamydosporia strain JL-1 fungal strains, are a biological alternative to reduce plant parasitic nematodes on the roots of plants. The objective of this research was to determine the most effective concentration of P. lilacinum strain PL-11 and P. chlamydosporia strain JL-1, for the management of root-knot nematode (Meloidogyne spp.). In the Plant Pathology laboratory, at University of Caldas, in a completely randomized design, 1 mL of P. lilacinum (1 x 10⁹ spores / mL) were added to 32 bacteriological Petri dishes with agar; subsequently, 16 of them were inoculated with a 30 mL suspension containing 10 eggs of P. chlamydosporia and Meloidogyne mayaguensis and, the other 16, were inoculated with a 30 mL suspension containing 10 juveniles (J2) of the two Meloidogyne species. Fungal infection of eggs and mortality of juveniles (J2) of the two species of Meloidogyne were evaluated at 24, 72, 120 and 168 h. The same procedure was performed with P. chlamydosporia, the combination P. lilacinum and P. chlamydosporia, P. lilacinum and P. chlamydosporia in combination with Carbofuran at concentrations between 1 x 10³ and 1 x 10⁹ spores / mL. The positive and negative controls were Carbofuran and water, respectively. Results demonstrated that mixing P. lilacinum and P. chlamydosporia (1 x 10⁶ spores / L) in combination with Carbofuran, and the mixture P. lilacinum and P. chlamydosporia (1 x 10⁸ spores / mL), caused the highest infections on eggs with 85% and 80%, respectively, and caused the highest mortality of juvenile (J2) of M. incognita and M. mayaguensis with 93% and 75%, respectively, compared to the water treated group, at 168 h.

Keywords : biological control; infection; Meloidogyne; Pochonia chlamydosporia; Purpureocillium lilacinum.

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