Abstract

ALONSO ESQUIVEL, Maruchi et al. Genetics variability estimation among coconut ecotypes in Cuba by ISTR. Rev. colomb. biotecnol [online]. 2008, vol.10, n.2, pp.6-13. ISSN 0123-3475.

The molecular characterization among 16 coconut ecotypes belonging to a population of Baracoa municipality, Guantanamo province, employing the ISTR technique (Inverse Sequence Tagged Repeat) has been done. The molecular analysis ISTR detected a polymorphism of 81.8%, showing the potenciality of this marker to do studies on molecular diversity in coconut. The identification percentage was high (P_i= 91.2%) suggesting that primer combinations employed could be used to ecotype identification studies on coconut populations of Baracoa. The heterocigocity hoped was low (H_e=0.30). Diversity evaluation in different coconut ecotypes by means of ISTR markers showed the variability level, according to the groups. This corresponds with the great morphologic variation observed in the population in situ. These results suggest that natural hybridization has been a determinant factor generating the variability found between the ecotypes, typical of coconut varieties.

Keywords : Variability; Cocos nucifera; molecular markers; polymorphism; heterozigosity.

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