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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475


CABRERA JOVA, Manuel et al. “Blanco de Guinea” (Dioscorea cayenensis - D. rotundata ) yam clone in vitro nodal segment multiplication in a temporary immersion system. Rev. colomb. biotecnol [online]. 2008, vol.10, n.2, pp.97-103. ISSN 0123-3475.

´Blanco de Guinea´ yam clone nodal segment multiplication was increased by using the temporary immersion system (TIS). The highest values for plant height, internodal number per plant and fresh and dry weight were obtained in this culture system. Nodal segments cultivated in TIS produced the highest multiplication coefficient (8,50). These plants were characterised by their green colour and rapid growth. The number of plants having vitrification symptoms (3,30) became reduced due to the favourable culture conditions created in the TIS. It is recommended that nodal segment multiplication in TIS be include in the available micropropagation protocols for Dioscorea cayenensis - D. rotundata to decrease propagation/production costs.

Keywords : temporary immersion system; liquid medium; micropropagation.

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