Abstract

PEDROZA-MANRIQUE, Jaime Alonso. The effect of activated charcoal, indol acetic acid (IAA) and benzylaminopurine (BAP) on Epidendrum elongatum Jacq protocorm-like body (PLB) development in in vitro conditions. Rev. colomb. biotecnol [online]. 2009, vol.11, n.1, pp.17-32. ISSN 0123-3475.

The vegetative development of protocorm-like bodies (PLB) from Epidendrum elongatum Jacq. (an endemic and endangered orchid) was effectively achieved through in vitro culture in Murashige and Skoog medium (1962); the effect of the interaction between the following three factors was studied: activated charcoal (0.0, 0.5, 1.0% (w/v)), indol acetic acid (0.0, 0.5, 1.0 mg.L^-1), benzylaminopurine (0.0, 0.5, 1.0 mg.L^-1). The culture medium being gerused was enriched with 3% sucrose and 0.1 g.L^-1 myo-inositol. This study revealed that the effect of 0.5% and 1.0% activated charcoal concentrations interacting with 0.5 mg.L^-1 AIA on PBL growth rate was positive for E. elongatum PBL development in vitro conditions, whereas the interaction of BAP (at both 0.5 mg.L^-1 and 1.0 mg.L^-1 concentration) did not produce such favourable results regarding the degree of development of the PLB being studied. This study describes a protocol leading to more than 270,000 seedlings enjoying excellent vegetative growth being obtained within 30 weeks from a single capsule from this important orchid. This protocol represents a model for conserving endangered species and promotes large-scale Epidendrum elongatum propagation.

Keywords : Orchids; Epidendrum elongatum; germination; in vitro; activated charcoal; IAA; BAP.

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