Abstract

PINZON GUTIERREZ, Yeimy Alexandra; BUSTAMANTE, Silvia Lizette  and  BUITRAGO, Gustavo. Evaluating methods for preserving yam (Dioscorea sp) phytopathogenic fungii. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.8-18. ISSN 0123-3475.

An essential element in phytopathogenic microorganism research is their preservation and safe use. The purpose of this study was to evaluate methods for preserving fungi producing diseases in yam, using potential viability, stability and the presence or absence of macro- and microscopic characteristic changes as response factors. More suitable methods for Colletotrichum and Fusarium genera were proposed as a result of the work; these are fungi causing the greatest losses in yam plantations on the Colombian Caribbean Coast. The strains used in this study came from collections kept at the University of Sucre and the Universidad Nacional de Colombia’s Institute of Biotechnology (IBUN) in Bogota and a donation from Dr Lucia Afanador from the Universidad Nacional in Medellín. These strains were subjected to different preservation methods, including cryopreservation, lyophilisation and periodic culture with and without mineral oil. The results led to choosing cryopreservation as the most efficient method for preserving the collection and the periodic culture with mineral oil as an alternative and complementary method. These minimised the risk of biological material loss and provided handling conditions conserving the biological characteristics of the fungi being studied from the fields of microbiology, biochemistry and molecular biology

Keywords : cryopreservation; lyophilisation; periodic culture; Colletotrichum sp; Fusarium sp.

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