Revista Colombiana de Biotecnología
versión impresa ISSN 0123-3475
CABRERA JOVA, Manuel et al. A protocol for yam (Dioscorea alata L.) microtuber formation in temporary immersion system. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp. 19-30. ISSN 0123-3475.
The following working objectives were defined for developing a protocol for ´Pacala Duclos´ yam clone (Dioscorea alata L.) microtuber formation in temporary immersion systems (TIS): time effect, immersion frequency and culture medium volume influence per in vitro cultivated plant. The results led to defining that the highest performance regarding microtuber number formed per plant was achieved with a 15-minute immersion time and six-hourly immersion frequency; the microtubers presented the greatest dry and fresh weight and largest diameter at such immersion time and frequency. Furthermore, the highest total number of microtubers per system and the largest number of microtubers usable for planting (vegetal propagation) material were obtained after 18 weeks culture. Regarding culture medium volume per plant, the greatest amount of usable microtubers was achieved with 60 ml per in vitro plant, microtubers presenting the highest dry matter content. As microtubers obtained in this type of TIS had fresh weights higher than 2.40 gFW they could be used as direct planting material in field conditions.
Palabras llave : microtuber; immersion and frequency time; culture medium volume.