Revista Colombiana de Biotecnología
versión impresa ISSN 0123-3475
ESQUIVEL, Maruchi Alonso et al. Characterising Cuban papaya accessions (Carica papaya L.) by AFLP markers. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp. 31-39. ISSN 0123-3475.
Molecular markers are valuable tools for genetic studies in plants and they are often used successfully in genetic breeding, mainly for choosing progenitors and selection. Polymorphism observed by amplified fragment length polymorphism (AFLP) has been useful for genetic diversity studies in fruit trees. Twelve papaya accessions from the Tropical Fruit Crop Research Institute germplasm bank were molecularly characterised by AFLP. 431 bands having 73.3% polymorphism were obtained using 6 primer combinations. The total number of band patterns identified was the same in all combinations assayed with a high percentage of identification, suggesting that such primer combinations could be used for genetic variability studies in papaya. The results demonstrated genetic diversity among the papaya accessions evaluated, indicating the origin of the analysed genotypes from exogenous material and open pollination of a selected group of material. It is thus recommended that local accessions and their selection be monitored as well as the introduction of new foreign genotypes as two ways of increasing the genetic diversity of the Cuban papaya germplasm bank.
Palabras llave : molecular marker; polymorphism; genetic diversity.