Abstract

BARRANCO OLIVERA, Luis A et al. The effect of inoculum density on embryogenic cell suspension proliferation and differentiation in banana hybrid cultivar FHIA-18 (Musa spp. AAAB). Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.40-48. ISSN 0123-3475.

An extremely useful tool for dealing with the enormous problems involved in banana growing (Musaceae) caused by the attack of diseases such as black Sigatoka can be obtained today by ensuring an efficient regeneobration system via somatic embryogenesis. The work was aimed at defining appropriate cell densities for embryogenic cell suspension growth stages and somatic embryo formation in liquid culture medium. Immature male inflorescence buds from Musa AAAB cf FHIA-18 were used as vegetal material. The results showed that it is possible to establish homogeneous cell suspensions from somatic embryos in globular stage and obtain greater cell biomass volume by multiplying the suspension with 3% sedimented cell volume (density). Embryos began to form structures in culture medium consisting of globular stage somatic proembryos and embryos from the fifteenth day onwards. The best results amongst the densities studied were obtained with 100 mgMF, in which 1,871 ES.l -1 were formed weighing 248 mgMF.l-1

Keywords : Somatic embryo; Musaceas; cell density.

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