Abstract

JIMENEZ BARRETO, Jenny Paola; CHAPARRO-GIRALDO, Alejandro  and  BLANCO, Jennifer. Evaluating different phytohormone combinations in regenerating Solanum tuberosum (Solanaceae) ar. Pastusa Suprema from intermodal explants. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.67-74. ISSN 0123-3475.

Plant regeneration using tissue culture represents an important biotechnological tool which is necessary in processes such as obtaining transgenic plants. An efficient regeneration system was established for Solanum tuberosum L. var. Pastusa Suprema; this specie is susceptible to being genetically transformed. The effect of zeatin riboside (ZR), gibberellic acid (GA3) and naphthalene acetic acid (NAA) phytohormones used in specific combinations was evaluated regarding callus induction and bud regeneration and number per explant. The presence of NAA was seen to be essential in explants’ callogenic and regenerative response. Adding 3.0 mg/L ZR, 0.02 mg/L NAA and 1.0mg/L GA3 to a basic M-S medium was an appropriate hormonal combination for inducing indirect organogenesis in the Pastusa Suprema potato variety; it produced more than 90% callus genesis and plant regeneration and an average of six regenerants per explant

Keywords : zeatin riboside; naphthalene acetic acid; gibberellic acid; indirect organogenesis.

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