Abstract

PEDROZA-MANRIQUE, Jaime Alonso  and  CABALLERO AREVALO, Mauricio. Evaluating the effect of MS medium and temperature in developing Marchantia polymorpha L. (Marchantiaceae) propagules in in vitro and ex vitro conditions. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.85-104. ISSN 0123-3475.

Marchantia polymorpha propagules were propagated in this research in in vitro conditions to assess their growth in terms of development time. Development rate and contamination regarding each treatment were also evaluated by weekly observations and crop records for each experimental unit per treatment. Talus shape and characteristics were also qualitatively evaluated by weekly observations and records. Fisher’s statistical test was used for analysing quantitative results by variance analysis with random design and 4 x 2 factorial arrangement. Appropriate conditions for cultivating M. polymorpha propagules were established at 25% Murashige and Skoog mineral salt concentration (1962), incubated at 25°C ± 1°C. After 13 weeks development in in vitro conditions, they were adapted to natural conditions by changing development site substrate and controlling temperature and humidity. Plant survival was evaluated for 10 weeks during this stage. Conditions were then changed and (as qualitative characteristic) talus vigour was taken into account. In was also determined that the ex vitro cultivation level should allow for gradual adjustment to new humidity, temperature and substrate conditions, taking special care that these conditions were not so altered as to become insurmountable. This pioneering M. polymorpha propagation protocol in Colombia is a model for the future maintenance and mass development of different bryophyte species, especially those which are endangered in our country

Keywords : bryophyte; in vitro culture; ex vitro culture.

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