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Revista Colombiana de Biotecnología

Print version ISSN 0123-3475

Abstract

BORGES GARCIA, Misterbino; ESTRADA ABEAL, Edil; PEREZ RODRIGUEZ, Idelisa  and  MENESES RODRIGUEZ, Silvio. Using different disinfection treatments on in vitro cultures of the “caraqueño” Dioscorea alata L. clone. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp.127-135. ISSN 0123-3475.

The in vitro culture technique consists of aseptically culturing an isolated plant portion in controlled environmental conditions for the cells to express their intrinsic and induced potential. This work was aimed at determining the effect of different sodium hypochlorite concentrations and immersion times on in vitro establishment of yam (Dioscorea alata L.) “caraqueño” clone primary explants. Disinfection varied by using different sodium hypochlorite concentrations (1.5%, 2% and 2.5%) during different immersion times (10, 20 and 30 min). Bacterial and fungal contamination percentages were evaluated after 7 days and de novo bud number, shoot length, leaf number, explant establishment and necrosis percentages were determined after 40 days. A totally randomised experimental design was applied, having one- and two-factor variance analysis; the Tukey test was used for comparing means at 5% significance level. The results showed that the most appropriate disinfection treatment for uninodal yam (D. alata L.) segments used 1.5% sodium hypochlorite during 30 min immersion; this was most effective for the in vitro establishment of yam (D. alata L.) clone “caraqueño” primary explants, having high survival percentage in ex vitro conditions.

Keywords : yam; plant tissue culture; sodium hypochlorite; in vitro establishment.

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