Revista Colombiana de Biotecnología
Print version ISSN 0123-3475
BUITRAGO, F et al. Comparative study of Chalcone synthase promoters across plant families. Rev. colomb. biotecnol [online]. 2009, vol.11, n.2, pp. 136-142. ISSN 0123-3475.
In the post - genomic era the understanding of gene regulation has become a challenge and a research priority. In this research, we performed a comparative study of the regulator sequences of the chalcone synthase gene across plant families. Twenty-two sequences of chalcone synthase promoters were compared considering three regulator Cis elements: G-Box, H-Box and TATA Box. Our results show that these Cis elements are conserved among species and even at the family level. However, in some species all of the Cis elements were not found, showing that the expression and regulation of these promoters via the Cis elements can be variable. Additionally, a comparison between promoters from a species with a chalcone synthase multigene family showed that the duplicate genes are variable in the composition of the Cis elements, suggesting that these genes could be expressing in different ways.
Keywords : Promoter; Chalcone synthase; Cis elements; Floral expression.